Journal: Acta Biochimica et Biophysica Sinica

Article Title: Resident CD24 + LCN2 + LPCs aggravate fibrosis and inflammatory progression via the recruitment of TPPP3 + COL10A1 + macrophages in NASH

doi: 10.3724/abbs.2025081

Figure Lengend Snippet: Validation of the fibrosis-related genes TPPP3 and COL10A1 in bulk RNA-seq datasets (A) The boxplot shows the differences in the expression of the TPPP3 gene at different stages of NASH development. Compared with that in F2, expression was significantly greater in F3 and significantly higher in F4 than in F3 [* P < 0.05, log (fold change) < 2; **P < 0.01, log (fold change) ≥ 2]. (B) The boxplot shows the expression differences of the COL10A1 gene at different stages of NASH development. Compared with that in F0 and F1, expression in F3 and F4 was significantly greater than that in F2 [*P < 0.05, log (fold change) < 2; **P < 0.01, log (fold change) ≥ 2]. (C) The relationships between the expression of TPPP3 and the expressions of marker genes of LPCs were measured via Pearson’s product-moment correlation analysis. (D) The relationship between the expression of COL10A1 and the expressions of marker genes of LPCs was measured via Pearson’s product-moment correlation analysis.

Article Snippet: The following primary antibodies were used: anti-collagen 10A1 antibody (1:1000; 15057-1-AP; Proteintech, Chicago, USA), anti-β-actin antibody (1:1000; #4970; Cell Signaling Technology, Boston, USA) and anti-TPPP3 antibody (1:1000; #72026; Cell Signaling Technology).

Techniques: Biomarker Discovery, RNA Sequencing, Expressing, Marker

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Resident CD24 + LCN2 + LPCs aggravate fibrosis and inflammatory progression via the recruitment of TPPP3 + COL10A1 + macrophages in NASH

doi: 10.3724/abbs.2025081

Figure Lengend Snippet: Expressions of TPPP3 and COL10A1 are significantly increased in areas of advanced fibrosis in the liver tissue of CDAHFD-induced NASH mice (A) qPCR results showing the expressions of fibrosis-related genes in CDAHFD-induced NASH model mice fed with a CDAHFD for various durations (in weeks). (B) Western blot analysis results for TPPP3 and COL10A1 expressions in CDAHFD-induced NASH model mice fed with a CDAHFD for various durations (in weeks). β-Actin was utilized as a loading control for accurate protein quantification and normalization. (C) Summarized western blot data showing the relative protein levels of TPPP3 and COL10A1 in CDAHFD-induced NASH mice fed with a CDAHFD for various durations (in weeks). “ns” indicates no statistical significance; *P < 0.05, **P < 0.01, and ****P < 0.0001. (D) Immunohistochemistry images showing TPPP3 and COL10A1 expression in CDAHFD-induced NASH mice fed with a CDAHFD for various durations (in weeks). Scale bar: 200 μm. The magnification of the picture is 100×.

Article Snippet: The following primary antibodies were used: anti-collagen 10A1 antibody (1:1000; 15057-1-AP; Proteintech, Chicago, USA), anti-β-actin antibody (1:1000; #4970; Cell Signaling Technology, Boston, USA) and anti-TPPP3 antibody (1:1000; #72026; Cell Signaling Technology).

Techniques: Western Blot, Control, Immunohistochemistry, Expressing

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Resident CD24 + LCN2 + LPCs aggravate fibrosis and inflammatory progression via the recruitment of TPPP3 + COL10A1 + macrophages in NASH

doi: 10.3724/abbs.2025081

Figure Lengend Snippet: Expressions of TPPP3 and COL10A1 are elevated in MASLD patients (A) qPCR analysis of liver tissues from healthy controls and MASLD patients revealed increased mRNA expressions of fibrosis-related genes, including TPPP3 and COL10A1, in MASLD. (B) Western blot analysis of liver lysates from healthy controls and MASLD patients revealed increased protein levels of COL10A1 and TPPP3 in MASLD. β-Actin was utilized as a loading control for accurate protein quantification and normalization. (C) Quantification of TPPP3 and COL10A1 protein expression relative to that of β-actin via western blot analysis. “ns” indicates no statistical significance; ***P < 0.001, and ****P < 0.0001. (D) Representative images of H&E staining and immunohistochemistry staining for TPPP3 and COL10A1 in liver tissue sections from healthy controls and MASLD patients. Increased staining intensity for TPPP3 and COL10A1 is observed in MASLD tissues. Scale bar: 200 μm. The magnification of the picture is 100×.

Article Snippet: The following primary antibodies were used: anti-collagen 10A1 antibody (1:1000; 15057-1-AP; Proteintech, Chicago, USA), anti-β-actin antibody (1:1000; #4970; Cell Signaling Technology, Boston, USA) and anti-TPPP3 antibody (1:1000; #72026; Cell Signaling Technology).

Techniques: Western Blot, Control, Expressing, Staining, Immunohistochemistry

Journal: Scientific reports

Article Title: TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma.

doi: 10.1038/s41598-023-45233-w

Figure Lengend Snippet: Figure 4. TPPP3 induced epithelial-mesenchymal transition by regulating the expression of Snail1 protein. (A) Western Blot analysis the effect of knocking down the expression of TPPP3 on the expression of common key proteins in the EMT process. (B) The effect of overexpression of Snail1 in cells with knockdown of TPPP3 on the expression of E-cadherin, N-cadherin and Vimentin, the key markers of EMT process. (C, D) Snail1 was overexpressed in TPPP3 knockdown cells, and the invasion ability of glioma cells was quantitatively analyzed by Transwell experiment. n = 5 each group. (E, F) The wound healing experiment tested the migration ability of glioblastoma cells after overexpression of Snail1 in TPPP3 knockdown cells. n = 5 each group. (G) The CCK8 experiment analyzed the effect of overexpression of Snail1 in TPPP3 knockdown cells on the proliferation of glioblastoma cells. (H) Flow cytometry analysis detected the apoptosis of glioblastoma cells after overexpression of Snail1 in TPPP3 knockdown cells. n = 4 each group. Data from at least three independent experiments were quantified. **P < 0.01, ***P < 0.001.

Article Snippet: The involved primary antibodies were as follow and concentration was diluted according to product instructions: TPPP3 (NBP2-13469, NBP2-95209, Novus Biologicals), and N-cadherin (ab76011, Abcam), E-cadherin (ab40772, Abcam), Vimentin (ab8978, Abcam), Snail1 (13099-1-AP, Proteintech), Slug (ab302780, Abcam), Twist1 (ab175430, Abcam), ZEB1 (ab203829, Abcam), β-catenin(ab203829, Abcam).

Techniques: Expressing, Western Blot, Over Expression, Knockdown, Migration, Flow Cytometry

Journal: Scientific reports

Article Title: TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma.

doi: 10.1038/s41598-023-45233-w

Figure Lengend Snippet: Figure 6. Data analysis of clinical significance of TPPP3 in glioblastoma. (A) Immunohistochemical images of representative glioblastoma tissues with high and low TPPP3 expression. (B) Correlation analysis between TPPP3 and Snail1 in glioblastoma tissues. (C, D) The expression level of TPPP3 in glioblastoma affected the survival of patients. HR, Hazard Ratio. ****P < 0.0001.

Article Snippet: The involved primary antibodies were as follow and concentration was diluted according to product instructions: TPPP3 (NBP2-13469, NBP2-95209, Novus Biologicals), and N-cadherin (ab76011, Abcam), E-cadherin (ab40772, Abcam), Vimentin (ab8978, Abcam), Snail1 (13099-1-AP, Proteintech), Slug (ab302780, Abcam), Twist1 (ab175430, Abcam), ZEB1 (ab203829, Abcam), β-catenin(ab203829, Abcam).

Techniques: Immunohistochemical staining, Expressing